Collagen protein of fibroblasts was detected by Western blotting. The release of IL-12 and TNF- αby macrophages was measured by ELISA assay, and Transwell assay was performed to analyze its migration and invasion. The association between p300 and miR-451b, miR-451b and RhoA was validated by luciferase reporter assay. Expression miR-451b and its potential transcription factor p300 were detected. Macrophages and lung fibroblasts were exposed to 10% cigarette smoke extract (CSE) solution for 24 h. However, the function and molecular mechanisms of miR-451b in the pathogenesis of COPD remain elusive.
A previous study identified miR-451b as a potential biomarker in smoker with or without chronic obstructive pulmonary disease (COPD).
We discuss these findings relative to challenges of developing efficient and effective staff-training tutorials.Background. Social-validity measures found the posttraining graphs to be significantly better than publication quality. All four technicians showed mastery-level graphing accuracy with accompanying increases in efficiency following training, which maintained at follow-up without direct feedback from an expert behavior analyst. We evaluated the effects of a video-modeling tutorial on graphing performance in Prism using a multiple-baseline design. Our review identified nine commonly recommended features, and Prism facilitated the creation of graphs with those features. In this investigation, we (a) define the desirable features of publication-quality, single-case design graphs based on recommendations in the scholarly literature, (b) suggest GraphPad Prism as a suitable graphing program for creating graphs with those features, and (c) develop and validate a video-modeling tutorial designed to train behavior technicians to create such graphs.
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